p53 prk5 vectors Search Results


hilymax  (Dojindo Labs)
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Dojindo Labs hilymax
Hilymax, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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snb hg14816 g software  (Sino Biological)
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Sino Biological snb hg14816 g software
Snb Hg14816 G Software, supplied by Sino Biological, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p53 prk5 vectors  (Addgene inc)
93
Addgene inc p53 prk5 vectors
P53 Prk5 Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcdna3 myc3 nrf2  (Addgene inc)
93
Addgene inc pcdna3 myc3 nrf2
Pcdna3 Myc3 Nrf2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ins1-cre mice  (BioResource International Inc)
90
BioResource International Inc ins1-cre mice
Ins1 Cre Mice, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pgl3basic-mdm2-t1 (addgene, plasmid# 32365)  (Addgene inc)
90
Addgene inc pgl3basic-mdm2-t1 (addgene, plasmid# 32365)
Pgl3basic Mdm2 T1 (Addgene, Plasmid# 32365), supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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prl-tk control plasmid  (Promega)
90
Promega prl-tk control plasmid
Prl Tk Control Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sulforaphane  (Millipore)
90
Millipore sulforaphane
(A) Effects of p53, p62 and NRF2 overexpression, NRF2 silencing and <t>sulforaphane</t> on Mdm2 promoter activity in transfected KPC cells (n = 3). (B) Chromatin immunoprecipitation assays probing NRF2 and small MAF protein recruitment to the MDM2 promoter in WT and NRF2 ablated MIA PaCa-2 (n = 3) cells. (C) Sphere formation of control or MDM2-ablated MIA PaCa-2 and Capan-2 cells with or without NICD1 overexpression. (D) SA-β-gal staining and γ-H2AX IB of cells as in C. (E) Sphere formation of control and p62-ablated MIA PaCa-2 and Capan-2 cells with or without MDM2 CRISPR-mediated activation vector (n = 3). (F) SA-β-gal staining and γ-H2AX IB of cells as in E. (G) Sphere formation of control and NRF2-ablated MIA PaCa-2 and Capan-2 cells with or without MDM2 CRISPR-mediated activation vector (n = 3). (H) SA-β-gal staining and γ-H2AX IB of cells as in G. (I) A scheme explaining how p62 accumulation, operating via the NRF2-MDM2 module leads to dedifferentiation and cell cycle progression in preneoplastic lesions in pancreas. Results are mean ± SEM; *, p < 0.05; **, p < 0.01; ***, p < 0.001 by Student’s t test. See also Figure S7.
Sulforaphane, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pgl3basic mdm2 t1  (Addgene inc)
88
Addgene inc pgl3basic mdm2 t1
(A) Effects of p53, p62 and NRF2 overexpression, NRF2 silencing and <t>sulforaphane</t> on Mdm2 promoter activity in transfected KPC cells (n = 3). (B) Chromatin immunoprecipitation assays probing NRF2 and small MAF protein recruitment to the MDM2 promoter in WT and NRF2 ablated MIA PaCa-2 (n = 3) cells. (C) Sphere formation of control or MDM2-ablated MIA PaCa-2 and Capan-2 cells with or without NICD1 overexpression. (D) SA-β-gal staining and γ-H2AX IB of cells as in C. (E) Sphere formation of control and p62-ablated MIA PaCa-2 and Capan-2 cells with or without MDM2 CRISPR-mediated activation vector (n = 3). (F) SA-β-gal staining and γ-H2AX IB of cells as in E. (G) Sphere formation of control and NRF2-ablated MIA PaCa-2 and Capan-2 cells with or without MDM2 CRISPR-mediated activation vector (n = 3). (H) SA-β-gal staining and γ-H2AX IB of cells as in G. (I) A scheme explaining how p62 accumulation, operating via the NRF2-MDM2 module leads to dedifferentiation and cell cycle progression in preneoplastic lesions in pancreas. Results are mean ± SEM; *, p < 0.05; **, p < 0.01; ***, p < 0.001 by Student’s t test. See also Figure S7.
Pgl3basic Mdm2 T1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 88 stars, based on 1 article reviews
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(A) Effects of p53, p62 and NRF2 overexpression, NRF2 silencing and sulforaphane on Mdm2 promoter activity in transfected KPC cells (n = 3). (B) Chromatin immunoprecipitation assays probing NRF2 and small MAF protein recruitment to the MDM2 promoter in WT and NRF2 ablated MIA PaCa-2 (n = 3) cells. (C) Sphere formation of control or MDM2-ablated MIA PaCa-2 and Capan-2 cells with or without NICD1 overexpression. (D) SA-β-gal staining and γ-H2AX IB of cells as in C. (E) Sphere formation of control and p62-ablated MIA PaCa-2 and Capan-2 cells with or without MDM2 CRISPR-mediated activation vector (n = 3). (F) SA-β-gal staining and γ-H2AX IB of cells as in E. (G) Sphere formation of control and NRF2-ablated MIA PaCa-2 and Capan-2 cells with or without MDM2 CRISPR-mediated activation vector (n = 3). (H) SA-β-gal staining and γ-H2AX IB of cells as in G. (I) A scheme explaining how p62 accumulation, operating via the NRF2-MDM2 module leads to dedifferentiation and cell cycle progression in preneoplastic lesions in pancreas. Results are mean ± SEM; *, p < 0.05; **, p < 0.01; ***, p < 0.001 by Student’s t test. See also Figure S7.

Journal: Cancer cell

Article Title: Stress Activated NRF2-MDM2 Cascade Controls Neoplastic Progression in Pancreas

doi: 10.1016/j.ccell.2017.10.011

Figure Lengend Snippet: (A) Effects of p53, p62 and NRF2 overexpression, NRF2 silencing and sulforaphane on Mdm2 promoter activity in transfected KPC cells (n = 3). (B) Chromatin immunoprecipitation assays probing NRF2 and small MAF protein recruitment to the MDM2 promoter in WT and NRF2 ablated MIA PaCa-2 (n = 3) cells. (C) Sphere formation of control or MDM2-ablated MIA PaCa-2 and Capan-2 cells with or without NICD1 overexpression. (D) SA-β-gal staining and γ-H2AX IB of cells as in C. (E) Sphere formation of control and p62-ablated MIA PaCa-2 and Capan-2 cells with or without MDM2 CRISPR-mediated activation vector (n = 3). (F) SA-β-gal staining and γ-H2AX IB of cells as in E. (G) Sphere formation of control and NRF2-ablated MIA PaCa-2 and Capan-2 cells with or without MDM2 CRISPR-mediated activation vector (n = 3). (H) SA-β-gal staining and γ-H2AX IB of cells as in G. (I) A scheme explaining how p62 accumulation, operating via the NRF2-MDM2 module leads to dedifferentiation and cell cycle progression in preneoplastic lesions in pancreas. Results are mean ± SEM; *, p < 0.05; **, p < 0.01; ***, p < 0.001 by Student’s t test. See also Figure S7.

Article Snippet: MIA PaCa-2 cells seeded in 24-well plates at a 2 × 10 4 cells/well were transfected with Lipofectamine 3000 reagent (Thermo Fisher Scientific, L3000015) with the following plasmids and sh-RNAs: pGL3Basic-Mdm2-T1 (Addgene, Plasmid# 32365) ( Chang et al., 2004 ) and its corresponding ARE mutant plasmid PGL3Basic-Mdm2-ARE-mut obtained by site-directed mutagenesis, pRL-TK control plasmid (Promega, E2241), WT p62 expression vector ( Umemura et al., 2016 ), pCDNA3-Myc3-Nrf2 (Addgene, Plasmid# 21555) ( Furukawa and Xiong, 2005 ) , Flag-p53/pRK5 (Addgene 39237) ( Jiang et al., 2011 ) , NRF2-shRNA (Santa Cruz, sc-37030-SH), Control-shRNA (Santa Cruz, sc-108060) or treated with 10 µM sulforaphane (Millipore Sigma, S4441) or DMSO as control.

Techniques: Over Expression, Activity Assay, Transfection, Chromatin Immunoprecipitation, Staining, CRISPR, Activation Assay, Plasmid Preparation